HW8-primers for detection and RE analysis
Požadavky na absolvování
Otevřené: úterý, 16. dubna 2024, 16.00
Termín: čtvrtek, 2. května 2024, 01.00
1)Find primers for the detection of your gene that would be specific (other transcript variants allowed).
2) Check the primers by multalin (CDS,mRNA and F, R)
3)Find out if and how many times cut your CDS following REs: EcoRI, XbaI, NcoI
4)Find out a RE that cuts just once and simulate the restriction digest of your CDS, what will be the products.