HW8-primers for detection and RE analysis
Požadavky na absolvování
Otevřené: úterý, 22. dubna 2025, 13.00
Termín: pátek, 2. května 2025, 01.00
1)Find primers for the detection of your gene that would be specific (other transcript variants allowed).
2) Check the primers by multalin (CDS,mRNA and F, R)
3)Find out if and how many times cut your CDS following REs: EcoRI, BamHI, XbaI
4)Find out an REs that cut just once and on that cuts twice. Simulate the restriction digest of your CDS by both enzymes, what will be the products?