HW8-primers for detection and RE analysis
Conditions d'achèvement
Ouvert le : mardi 16 avril 2024, 16:00
À remettre : jeudi 2 mai 2024, 01:00
1)Find primers for the detection of your gene that would be specific (other transcript variants allowed).
2) Check the primers by multalin (CDS,mRNA and F, R)
3)Find out if and how many times cut your CDS following REs: EcoRI, XbaI, NcoI
4)Find out a RE that cuts just once and simulate the restriction digest of your CDS, what will be the products.