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DL 1
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  1. Introduction to applied bioinformatics
  2. Topic 8_Nucleotide bioinformatics IV
  3. HW8-primers for detection and RE analysis

HW8-primers for detection and RE analysis

Abschlussbedingungen
Opened: Mittwoch, 8. April 2026, 13:19
Due: Mittwoch, 6. Mai 2026, 04:19

1)Find primers for the detection of your gene that would be specific (other transcript variants allowed).

2) Check the primers by multalin (CDS,mRNA and F, R)

3)Find out if and how many times cut your CDS following REs: EcoRI, BamHI, XbaI
 
4)Find out an REs that cut just once and one that cuts twice. Simulate the     restriction digest of your CDS by both enzymes, what will be the products?

 

◄ Ex:Primers verification
Lesson 8 -pdf ►
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